Detection of Glucose Levels Using Excitation and Difference Raman Spectroscopy at the IUSL

Raman spectroscopy such as spontaneous Raman spectroscopy, coherent anti-stokes Raman spectroscopy, and stimulated Raman gain spectroscopy are widely used in physics, chemistry and biology fields for investigations of vibrational modes. To our best knowledge, very few applications of Raman spectroscopy for the in-vitro and invivo patient medical diagnostics have been reported because of low signal levels and strong background levels. The conventional Raman techniques for detection of body level constituents are generally associated with long exposure time (several ten minutes), high power laser pump fluency which is much above the safety limitation of the laser illumination for human body applications, and strong noise background. Conventional Raman is too weak to determine analyte (glucose) level in humans due to the background.